鈥� Objective.听
鈥擳o assess the reliability and usefulness of a new sandwich enzyme-linked immunoassay (ALZ-EIA) that detects Alzheimer's disease-associated proteins in the diagnosis of Alzheimer's disease.
Design.听
鈥擳he reliability of the assay was assessed between two laboratories. Sensitivity and specificity of a diagnostic algorithm based on the results of the ALZ-EIA were determined using the Consortium to Establish a Registry for Alzheimer's Disease neuropathological diagnoses as the "gold standard."
Setting.听
鈥擜utopsy cases were obtained from a teaching hospital with a specialized Alzheimer Disease Diagnostic and Treatment Center.
Cases.听
鈥擝rain tissue was selected from 24 cases with dementia and 10 normal controls.
Main Outcome Measures.听
鈥擮ptical density measurements from the ALZ-EIA in the hippocampus and three neocortical regions.
Results.听
鈥擜 95% concordance in ALZ-EIA activity was found between the two laboratories, and an 85% concordance was found between ALZ-EIA and the Consortium to Establish a Registry for Alzheimer's Disease diagnoses. Perfect agreement was obtained for "typical" Alzheimer's disease cases (those with plaques and tangles), while discrepancies occurred for "atypical" cases (those with predominantly plaques or tangles).
Conclusions.听
鈥擳he ALZ-EIA provides a highly reliable method of assessing neurofibrillary degeneration. Its clinical usefulness as a diagnostic test would be enhanced by the availability of a complementary assay for 尾-amyloid.